Coding
fuGFP-CBDc

Part:BBa_K4488008:Design

Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li   Group: iGEM22_Sydney_Australia   (2022-09-30)
Revision as of 05:00, 9 October 2022 by Jali2097 (Talk | contribs) (→‎Design Notes)

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Fusion of free-use GFP with CBDcex (cellulose-binding domain) at the C-terminal end


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The construct has a constitutive RBS (BBa_B0034). 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDcex, similar to BBa_K1321342, to act as a small linker.

Source

The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321342) developed by the 2014 Imperial team Aqualose with the type of GFP replaced. The fuGFP sequence can be found here (BBa_K3814004).

References