Coding
mleR

Part:BBa_K4137002

Designed by: Jen-Hao Yao   Group: iGEM22_GEMS_Taiwan   (2022-08-08)
Revision as of 04:38, 9 October 2022 by Andreahuang (Talk | contribs)


Malate-binding Transcriptional Activator + 6xHis

BBa_K4137002, nicknamed mleR for the purposes of our project, is a malate-binding transcriptional activator belonging to l. lactis that has a 6xHis tag attached to permit protein purification. The mleR transcriptional activator portion of the part is responsible for governing malolactic fermentation in l. lactis, and responds to malate to induce malolactic enzyme production.

Fig.1 ccdA with C-Terminal 6x His-Tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None