Composite

Part:BBa_K4137007

Designed by: Andrea Huang   Group: iGEM22_GEMS_Taiwan   (2022-08-19)
Revision as of 04:13, 9 October 2022 by Andreahuang (Talk | contribs)


pLac+RBS+CcdB-Myc+B1006

This part consists of a pLac promoter, RBS (AAGGAG), a ccdB-Myc coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability.

Construct Design

We attached a Myc-Tag downstream of the ccdB sequence for purification purposes. A pLac promoter is attached upstream to the side of the open reading frame + RBS. The terminator BBa_B1006 is attached downstream of the sequence.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 271


[edit]
Categories
Parameters
None