Coding
CcdA

Part:BBa_K4137000

Designed by: Clifford S Chang   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 04:07, 9 October 2022 by Andreahuang (Talk | contribs)


CcdA-6xHis Antitoxin

BBa_K4137000 codes for the CcdA protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdA binds with CcdB which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. A 6xHis tag was added for protein purification and identification.


Fig.1 ccdA with C-Terminal 6x His-Tag.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None