Part:BBa_K4242011

Ppel+RBS+gfp+TT(pHYD-1)

Uses a factor Ppel(BBa_K4242001), strong RBS(BBa_J34801), gfp coding region(BBa_E0040), and TT(BBa_B0015). This part is an easy factor assessment tool. The GFP reporter enables you to find out whether the factor Ppel affects your works.

Usage and Biology

Promotor Ppel is a constitutive promotor from P. aeruginosa than can drive the transcription of downstream gene in the absence of its repressor FleQ. However, we don’t know whether it work well in other organisms. To test that, we constructed this part by combining Ppel with the reporter gene gfp. After our test, we found that this part doesn’t work well in E. coli and S. oneidenisis. No fluorescence difference was observed between the control group and pHYD-1 strain.

pHYD-1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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