Coding

Part:BBa_K4440001

Designed by: Viacheslav Kiselev   Group: iGEM22_Estonia_TUIT   (2022-10-08)
Revision as of 23:33, 8 October 2022 by Registry (Talk | contribs)

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preproSP-4B4-AGA2-Tyr1-3HA

These five protein modules (SP, 4B4 peptide, Aga2, Tyr1, 3HA tag) are fused together by flexible linkers. Signal peptide from the alpha factor directs the engineered protein to the secretory pathway. Once secreted, Aga2 anchors the protein to the cell wall. 4B4 peptide is responsible for the binding of the synthesized melanin to accumulate it on the cell surface (Ballard et al., 2011). 3HA tag is used to later test the presence of the enzyme on the cell surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None