Part:BBa_K4432020
CrtW: β−carotene ketolase
This part is an Escherichia coli codon optimized version of the ß-carotene-ketolase (CrtW148) from Nostoc punctiforme PCC 73102 (NCBI Reference Sequence: WP_012412154.1).
Usage and Biology
The ß-carotene-ketolase (EC 1.14.99.63) catalyses the conversion of ß-carotene into echinenone and of echinenone into canthaxanthin (Figure 1).
The Nostoc punctiforme CrtW [1] is a 30.743 kDa protein of 262 amino acids.
When placed under the control of the hybrid T5 promoter regulated by LacI (BBa_K4432000) or the classical pLac promoter (BBa_R0010), this part leads to the synthesis of canthaxanthin in an E. coli producing ß-carotene.
For further details, see the Main Pages on the Registry of the CrtW expression plasmids (BBa_K4432120, BBa_K4432220 and BBa_K4432320).
References
[1] Steiger S, Sandmann G. Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin. Biotechnology Letters (2004) 26: 813–817.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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