Part:BBa_K228876

AND GATE AraC+RBS(J44001)+T7ptag+Sal+SupD

This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 3686
  Illegal SpeI site found at 1343
  Illegal PstI site found at 1644
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3686
  Illegal NheI site found at 1355
  Illegal NheI site found at 1378
  Illegal SpeI site found at 1343
  Illegal PstI site found at 1644
  Illegal NotI site found at 3692
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3686
  Illegal BglII site found at 576
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 3686
  Illegal SpeI site found at 1343
  Illegal PstI site found at 1644
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 3686
  Illegal XbaI site found at 3701
  Illegal SpeI site found at 1343
  Illegal PstI site found at 1644
  Illegal NgoMIV site found at 408
  Illegal AgeI site found at 1093
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1096
  Illegal BsaI.rc site found at 2725