![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Part:BBa_K4212049:Design
Ger1
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3591
Illegal BglII site found at 3123
Illegal XhoI site found at 713 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542
Illegal NgoMIV site found at 66 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2778
Design Notes
PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. However, we planned to engineer the receptor to make it more sensitive to N-acetylglucosamine (NAG) - the chitin monomer. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein. We included the operon for germinant receptor GerA because GerA is responsive to L-alanine and responsible for B.subtilis germination. We incorporated GerA into our Golden Gate construct to create the GerAK chimera, as the model our dry lab predicted.
Source
Synthetic construct.
References
[1] https://parts.igem.org/Part:BBa_K2232020 [2] Ramírez-Guadiana, F.H., Meeske, A.J., Wang, X., Rodrigues, C.D.A. & Rudner, D.Z. (2017) The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation. Molecular Microbiology. 105 (5), 689–704. doi:10.1111/mmi.13728.