Composite

Part:BBa_K4268010:Experience

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski     Group: iGEM22_SUNY_Oneonta   (2022-09-30)
Revision as of 18:13, 8 October 2022 by Jazmine124 (Talk | contribs)


Level 0 parts BBa_R0085, BBa_B0034, BBa_K4268002 (Capsid Protein), and BBa_B0015 were cloned into the level 1 Golden Gate vector, PSB1K02. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.


Applications of BBa_K4268010

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