Coding
Part:BBa_K4461010
Designed by: CHANG, YU-TANG Group: iGEM22_NYCU-Taipei (2022-10-08)
Mut-mCherry + EcoRI and HindIII cutting site
To insert other proteins that can fusion with mCherry (BBa_J18932), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |