Part:BBa_K4268004:Experience

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of four colonies (clones) suspected of coating the insert Tail Tubular Protein A insert. The insert length is 615bp and the predicted size of the PCR product when using VF/VR primers is 920bp.

The gel indicates that all four colonies are likely to contain the correct insert and thus, the Tail Tubular Protein A was successfully cloned into a Level 0 Golden Gate Assembly basic part.

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