Coding

Part:BBa_K4339003

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-08)
Revision as of 16:24, 8 October 2022 by ChloeS (Talk | contribs)


MaSp1_K3264003mod_K3264002

A major ampullate spidroin 1 (MaSp1) based protein consisting of the Great Bay 2019 part BBa_K3264003 fused to K3264002. Due to the highly repetitive nature of spidroin proteins, the DNA coding sequences are also highly repetitive. Unfortunately we were unable to submit the original Great Bay sequence for synthesis, and therefore K3264003 had to be modified to allow for synthesis.


Usage and Biology

Sequence and Features

The sequence originates from the African nursery web spider Euprosthenops australis (Stark et al. 2007).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Stark, M. et al. (2007) ‘Macroscopic fibers self-assembled from recombinant miniature spider silk proteins’, Biomacromolecules, 8(5), pp. 1695–1701. Available at: https://doi.org/10.1021/bm070049y.


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