Part:BBa_K4390017

SUMO tag :: Glycine-Serine Linker

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM. This is a Level 0 part of type O part generating the following 4 base overhangs at upstream (AGCC) and downstream (TTCG) ends.

Usage and Biology

SUMO tag is a small protein with around 12 kDa. When expressing desired protein in E. coli, most protein will be insoluble which cause aggregation or instability. One solution to this situation is the fusion of desired protein with different tags or proteins which can increase the solubility. This part is a SUMO tag with a Glycine-Serine (GS) linker added at the 3’ end. SUMO tag is important to keep the stability (Henley, J. M. et al., 2007) and increase the solubility (Kuo, D. et al., 2014) in both prokaryote and eukaryote. The GS linker will be used to fuse the SUMO with metallothionein (Li, X. et al., 2021) to increase the protein stability and solubility in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Henley, J. M. et al. (2007) SUMOylation regulates kainate-receptor-mediated synaptic transmission. Nature. 447 (7142), 321–325.

Kuo, D. et al. (2014) SUMO as a Solubility Tag and In Vivo Cleavage of SUMO Fusion Proteins with Ulp1. Methods in molecular biology (Clifton, N.J.). 117771–80.

Li, X. et al. (2021) Genetic modifications of metallothionein enhance the tolerance and bioaccumulation of heavy metals in Escherichia coli. Ecotoxicology and environmental safety. 222112512–112512.