Coding

Part:BBa_K4339001

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-08)
Revision as of 15:39, 8 October 2022 by ChloeS (Talk | contribs)


Alanine tRNA synthetase

Alanine tRNA synthetase is an enzyme that catalyses the binding of alanine to tRNA. Modelling demonstrated that the expression of spider silk proteins (spidroins), which have a number of repetitive alanine regions, would be increased with the co-expression of alanine tRNA synthetase.


Usage and Biology

Sequence and Features

The sequence originates from E. coli MG1655 K-12 (Puteny et al. 1981)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
    Illegal AgeI site found at 709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1090
    Illegal SapI.rc site found at 1138
    Illegal SapI.rc site found at 1288

References

Putney, S.D., Sauer, R.T. and Schimmel, P.R. (1981) ‘Purification and properties of alanine tRNA synthetase from Escherichia coli. A tetramer of identical subunits.’, Journal of Biological Chemistry, 256(1), pp. 198–204. Available at: https://doi.org/10.1016/s0021-9258(19)70119-7.

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Categories
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