Part:BBa_K4239001
Enhanced luciferase subunits fiatluxC
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1311
Description
fiatluxC is made to be used with fiatluxD and fiatluxE. It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.
The systeme fiatluxC/fiatluxD/fiatluxE is made to be used with fiatluxA and fiatluxB, gathered in the fiatluxCDABE operon.
Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.
The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.
Construction
fiatluxC was directly synthesized with fiatluxD and the promoter J23117 (BBa_J23117). The description of the synthesis is on the page part (BBa_K4239006).
METTRE LES PRIMERS UTILISés| protein | fiatluxC |