Composite

Part:BBa_K238018

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-09-22)
Revision as of 13:02, 22 September 2009 by AnneV (Talk | contribs)

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EF ligation with promotor and terminator

this is a testing device for the formation of vanillin from ferulic acid. part K238006 is the testing device with the lock key system. in this part a promoter was put before part K238017.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 443
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 594
    Illegal AgeI site found at 1131
    Illegal AgeI site found at 1592
    Illegal AgeI site found at 2034
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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