Device

Part:BBa_K228866:Design

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-22)
Revision as of 10:14, 22 September 2009 by Spring zhq (Talk | contribs) (→‎Design Notes)

AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2007
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1839
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323


Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.

Source

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.

References