Regulatory

Part:BBa_K4325010

Designed by: Minchun He   Group: iGEM22_SZPT-CHINA   (2022-09-16)
Revision as of 14:34, 8 October 2022 by Bozaigao (Talk | contribs)


pDawn without LVA tag

Description

Our reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525

2022 SZPT-China

Biology

Our goal is to reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI as increasing the concentration of cI could enhance the repression of PR promoter.

S2.</p>

Figure 1. Sequence alignment of pDawn(cI-LVA) before and after removing LVA tag

[edit]
Categories
Parameters
None