Composite

Part:BBa_K228865

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-22)
Revision as of 10:05, 22 September 2009 by Registry (Talk | contribs)

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pSal-rbs-PhiR73 ( amber mutation )

T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apSal-rbs-PhiR73 ( amber mutation )