DNA

Part:BBa_K4361015

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-08-21)
Revision as of 14:19, 8 October 2022 by Larsvdb (Talk | contribs)


BlcR-binding oligo, 71 bp, IR1 + IR2 + IR1

Based off of BBa_K4361001, but inserting an additional "tca + IR1" after inverted repeat pair 2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and biology

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Results

~~~
Figure 2. Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with Part:BBa_K4361000 (scrambled oligo, negative control), the second bar and top dashed line correspond to those with Part:BBa_K4361001 (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.


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Categories
//awards/part_collection
Parameters
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