Regulatory

Part:BBa_I13453

Designed by: jkm   Group: iGEM04_MIT   (2005-05-25)
Revision as of 01:50, 30 April 2006 by Registry (Talk | contribs)

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Pbad promoter

PBad promoter from I0500 without AraC.

Usage and Biology

Has been used as a second promoter in a system containing I0500 (PBad+AraC). In this system, it showed behavior qualitatively indistinguishable from the I0500 copy of PBad. Has not been tested independent of AraC. A second part, I13458, should allow decoupling of PBad and AraC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

negative_regulators-NA-
positive_regulators1
[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/uncategorized
Parameters
negative_regulators
positive_regulators1