Regulatory

Part:BBa_K228009

Designed by: Gao Rencheng   Group: iGEM09_PKU_Beijing   (2009-09-09)
Revision as of 09:55, 22 September 2009 by Supergrc (Talk | contribs)

AraC protein(reversed sequence) and Pbad promoter

This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.

This promoter is placed upstream of a GFP gene to character its induction curve. We cultured the bacteria until the value of OD600 reached 0.4 and then added different concentration of arabinose. The process of inducement lasted two hours. We used flowcytometry to test the fluorescence in order to acquire the induction curve, which is shown in the below graph:

PKU AraC.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aAraC protein(reversed sequence) and Pbad promoter