Regulatory

Part:BBa_K228009

Designed by: Gao Rencheng   Group: iGEM09_PKU_Beijing   (2009-09-09)
Revision as of 09:45, 22 September 2009 by Supergrc (Talk | contribs)

AraC protein(reversed sequence) and Pbad promoter

This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.

This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer arabinose. The Induction curve is shown below:

PKU AraC.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAraC protein(reversed sequence) and Pbad promoter