Regulatory

Part:BBa_K4207013

Designed by: Jesper Mickos   Group: iGEM22_Aboa   (2022-09-30)
Revision as of 13:41, 8 October 2022 by Jjmick (Talk | contribs)


BYDV toehold switch B69 by TrigGate

Toehold switch for the detection of BYDV genomic RNA, designed in collaboration with TrigGate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

This toehold switch was designed according to the B-series ideal structure from Pardee et al. (2016). This structure was improved from the original toehold switch structure (Green et al., 2014) to reduce translational leakage. We screened the BYDV genome for conserved sequences. Each sequence was divided into 36-nucleotide long subsequences and we designed toehold switches designed to specifically bind to the sequence. This toehold switch was designed using the 30-nucleotide linker found in the 27B sensor (Pardee et al., 2016). We assigned a score for each toehold switch based on the three-parameter fit from Ma et al. (2018) and selected the best-ranking toehold switches for our library.


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