Coding

Part:BBa_K4345012

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 13:12, 8 October 2022 by LVdB (Talk | contribs)


FdeR

Coding sequence of fdeR from PfdeAR-FdeR biosensor circuit's detector module. FdeR is a part of Naringenin-Responsive transcriptional biosensor develop by De Paepe et al. (2018). Native naringenin-responsive regulatory circuit, LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae was redesigned to study the naringenin response in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 466
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 665
    Illegal NgoMIV site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419

[edit]
Categories
//cds
Parameters
None