Composite

Part:BBa_K4193030:Design

Designed by: Xinchen Yue   Group: iGEM22_OUC-China   (2022-10-08)
Revision as of 11:15, 8 October 2022 by NTSay (Talk | contribs) (→‎References)

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kill switch 2-L1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
    Illegal AgeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the kill switch contains YopE mRNA, which is a cytotoxin form Yersinia, and a ribozyme riboswitch. When no target molecule binds to the aptamer sequence, YopE is expressed and kills the cell, whereas the hammerhead ribozyme sequence degrades YopE mRNA.


Source

synthesize from company

References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.