Composite

Part:BBa_K4193026:Design

Designed by: Xinchen Yue   Group: iGEM22_OUC-China   (2022-10-08)
Revision as of 10:46, 8 October 2022 by NTSay (Talk | contribs)


QS system


Assembly Compatibility:
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    Illegal EcoRI site found at 5357
    Illegal EcoRI site found at 5682
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    Illegal XbaI site found at 3040
    Illegal XbaI site found at 3124
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Design Notes

we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription.

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.