Part:BBa_K4147006:Design

Disulfide interchange protein DsbA from Pseudomonas aeruginosa

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 219
  Illegal NgoMIV site found at 403
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Although signal peptides have been successfully used for this purpose, their presence does not always ensure the efficient secretion of protein into the periplasmic space and the formation of soluble, functional proteins that are correctly folded [2].

Source

The CDS comes from the GenBank accession number (NC_002516.2).

References

[1] Shouldice, S. R., Heras, B., Jarrott, R., Sharma, P., Scanlon, M. J., & Martin, J. L. (2010). Characterization of the DsbA Oxidative Folding Catalyst from Pseudomonas aeruginosa Reveals a Highly Oxidizing Protein that Binds Small Molecules. Antioxidants & Redox Signaling, 12(8), 921–931. doi:10.1089/ars.2009.2736 [2] Zhang, W., Lu, J., Zhang, S., Liu, L., Pang, X., & Lv, J. (2018). Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study. Microbial Cell Factories, 17(1). doi:10.1186/s12934-018-0894-y