Regulatory

Part:BBa_K4271010

Designed by: Ethan Ho   Group: iGEM22_Wego_Taipei   (2022-10-05)
Revision as of 09:33, 8 October 2022 by EthanClarke (Talk | contribs)


rpoD sigma factor



The rpoD sigma factor is a factor under the sigma-70 family and is a common sequence in E. coli. Activated by polyphosphate bondage, the sigma factor will be expressed and assist the RNA polymerase to bind to downstream ureA promoter (BBa_K4271011), thus expressing the mCherry (BBa_J18932) fluorescent protein for detection to determine engineering success. The usage of the rpoD sigma factor can be seen in BBa_K4271013 and BBa_K4271014.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 693
    Illegal BglII site found at 741
    Illegal BamHI site found at 1448
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1519


[edit]
Categories
Parameters
None