Composite
Part:BBa_K4165232
Designed by: Omnia AlaaEldeen Abdelazeem Group: iGEM22_CU_Egypt (2022-10-08)
Revision as of 08:55, 8 October 2022 by Omnia Alaa11 (Talk | contribs)
(GST) COH2-linker(G4S)-TD28REV
This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), GST (BBa_K4165070), COH2 (BBa_K4165003), G4S linker (BBa_K4165068), TD28REV (BBa_K4165006), Terminator (BBa_K731721), The GST tag was attached to the (COH2-Linker-TD28REV) coding sequence to serve in the purification using Glutathione resin column.
Usage and Biology
The main function is the binding of the tau-binding peptide with the Cohasin protein. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis.
Source
synthesized
Dry lab
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 45
Illegal SapI.rc site found at 189
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Categories
Parameters
None |