Composite

Part:BBa_K4137009:Design

Designed by: Andrea Huang   Group: iGEM22_GEMS_Taiwan   (2022-10-07)
Revision as of 07:22, 8 October 2022 by Stuberosum ryan (Talk | contribs) (→‎Source)


T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1523
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1036
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.


Source

https://parts.igem.org/Part:BBa_K1659003

References