Composite
Part:BBa_K4425009
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
pNEG
Express Taq polymerase when co-transform with trp repressor that cannot bind to its ligand.
Usage and Biology
Inactive trp repressors or trp repressor without its ligand can induce pNEG to express Taq polymerase. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 93
Illegal XbaI site found at 85
Illegal PstI site found at 1690 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 93
Illegal NheI site found at 2136
Illegal PstI site found at 1690 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 93
Illegal BglII site found at 82
Illegal BamHI site found at 1871
Illegal XhoI site found at 424
Illegal XhoI site found at 1501
Illegal XhoI site found at 2458 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 93
Illegal XbaI site found at 85
Illegal PstI site found at 1690 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 93
Illegal XbaI site found at 85
Illegal PstI site found at 1690
Illegal NgoMIV site found at 1525 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2014
Illegal BsaI.rc site found at 2254
Illegal SapI site found at 1326
Illegal SapI site found at 2394
Illegal SapI.rc site found at 1716
Illegal SapI.rc site found at 2304
Reference
Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.
[edit]
Categories
Parameters
None |