Coding

Part:BBa_K4193006:Design

Designed by: Rui Shi   Group: iGEM22_OUC-China   (2022-09-29)
Revision as of 06:59, 8 October 2022 by NTSay (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


AtCRE1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal BglII site found at 166
    Illegal BglII site found at 1102
    Illegal BglII site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

a cytokinin receptor from Arabidopsis thaliana

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.