Inverter

Part:BBa_K4425007

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)
Revision as of 06:47, 8 October 2022 by SeanHANPT (Talk | contribs)

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inverter based on λ repressor

An inverter based on λ repressor and lambda promoter


Usage and Biology

The inverter is assembled in pPOS. when trp repressor bind to trp, the expression of lambda repressor will be repressed, and the promoter expression of the downstream lambda promoter will be excited to produce more Taq polymerase.

Source

lambda repressor is from bacteriophage lambda and trp operator is generated by Ellefson et al.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 715
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
    Illegal BamHI site found at 507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 715
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

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Categories
Parameters
None