Inverter
Part:BBa_K4425007
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
inverter based on λ repressor
An inverter based on λ repressor and lambda promoter
Usage and Biology
The inverter is assembled in pPOS. when trp repressor bind to trp, the expression of lambda repressor will be repressed, and the promoter expression of the downstream lambda promoter will be excited to produce more Taq polymerase.
Source
lambda repressor is from bacteriophage lambda and trp operator is generated by Ellefson et al.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 715
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
Illegal BamHI site found at 507 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 715
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 715
- 1000COMPATIBLE WITH RFC[1000]
Reference
Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.
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Categories
Parameters
None |