Coding
Part:BBa_K4425006
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
pET28a-RBS-PtUGT-RBS-FMO
Co-express of glucosyltransferase PtUGT and monooxygenase FMO
Usage and Biology
We construct plasmid pET28a-FMO-PtUGT to co-express PtUGT and FMO to convert indole or 6-Br-indole to indican or 6-Br-indican respectively.
Source
BBa K4425006 is composited by part BBa_K4425002 from P. tinctorium<i/> and part BBa_K1131000 from <i>Methylophaga aminisulfidivorans
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1427
Illegal BamHI site found at 1398 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2318
Illegal AgeI site found at 2525 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1916
Reference
Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.
[edit]
Categories
Parameters
None |