Coding

Part:BBa_K4199002

Designed by: Yongzhu Li   Group: iGEM22_NWU-CHINA-A   (2022-09-30)
Revision as of 06:19, 8 October 2022 by Liyaa (Talk | contribs)


HrtR

The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 249
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None