Regulatory

Part:BBa_K228004

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-18)
Revision as of 15:24, 20 September 2009 by Maven (Talk | contribs)

NahR( reverse) - salicylate promoter

This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.

Note: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.

This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer salicylate. The Induction curve is shown below:

PKU Salicylate.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisshown to work in DH5α
copies~5
input_ssalicylate molecules
proteinNahR
signalling_moleculesalicylate molecules
switch_point3.32μM for inducing of 2 hrs