Composite

Part:BBa_K4137009

Designed by: Andrea Huang   Group: iGEM22_GEMS_Taiwan   (2022-10-07)
Revision as of 02:38, 8 October 2022 by Andreahuang (Talk | contribs)


T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006

This part consists of a T7 strong promoter, RBS (CTCCTT), a motor protein sequence yebF (BBa_K2922002), a GS Linker (BBa_J18921), a Chi19H8 coding sequence, a 6xHis tag (GTG x6), and a terminator (BBa_B1006). This part synthesizes and secretes a novel bacterial chitinase Chi18H8, which can be released extracellularly through e.coli Sec pathway (SecB-SecA-SecYEG, OmpF). This system continuously promotes the overexpression of Chi18H8 to lyse the chitin cell wall of the fungus fusarium oxysporum f.sp cubense, inhibiting fungal proliferation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1523
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1036
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None