Protein_Domain

Part:BBa_K4137011

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-10-06)
Revision as of 02:09, 8 October 2022 by AnneDYK (Talk | contribs)


CcdB coding sequence + 6xHis

The above sequence encodes for the CcdB protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdB binds with CcdA which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 234


[edit]
Categories
Parameters
None