Coding

Part:BBa_K4214001

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-07)
Revision as of 01:03, 8 October 2022 by Registry (Talk | contribs)

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Gastric Intrinsic Factor with signal peptide and polyhistidine tag

Gastric Intrinsic Factor with signal peptide and polyhistidine tag. The signal peptide is 22 AA long and allows for the secretion of the protein in the body.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
    Illegal NgoMIV site found at 14
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 353
    Illegal BsaI.rc site found at 1033
    Illegal SapI site found at 1023


[edit]
Categories
Parameters
None