Reporter
BFP

Part:BBa_K4321001

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 22:06, 7 October 2022 by Kjanneh (Talk | contribs) (→‎Reference)

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BFP

Usage and Biology

This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP). This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

BFP sequence and map. SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=BFP

Education in microscopy and digital imaging. ZEISS Microscopy Online Campus | Jellyfish Fluorescent Proteins. (n.d.). Retrieved October 4, 2022, from https://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps.html


[edit]
Categories
//cds/reporter
//function/reporter/fluorescence
Parameters
color