Part:BBa_K4180002

snf1Δ2-306aa N-truncated

snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation takes place on Thr210 to activate the catalytic activity of SNF1

Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200

Sequence and Features

        BBa K4180002
        snf1Δ2-306aa
         PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa
         PCR product

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]