Part:BBa_K4180003

snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)
C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Sequence and Features

       BBa K4180003 snf1Δ381-633aa
      PCR product on lane 3 compared to
       BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

        After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa
         PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a comparison

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Unknown
• 12
  INCOMPATIBLE WITH RFC[12]

  Unknown
• 21
  INCOMPATIBLE WITH RFC[21]

  Unknown
• 23
  INCOMPATIBLE WITH RFC[23]

  Unknown
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]