Composite

Part:BBa_K4140027

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-28)
Revision as of 15:47, 7 October 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


Permeability device

Part Description

In this composite Lac promoter regulate the expression of phenylalanine permease that is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.

Usage

We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease that is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.

Right

Figure 1. (shows the permeability circuit in our whole cell-based biosensor.)

Right

Figure 2. (shows the whole cell-based biosensor.)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None