Part:BBa_K4165220
Coh2 linked with tau binding peptide (TD28rev) & GSGS linker
Fusion protein consists of Tau binding peptide (TD28rev) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.
Usage and Biology
Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim, we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregation in our updated protac version system "Snitch system". Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
3D Modeling and Ranking
This fused protein was modeled using Modeller software and after running our quality assessment code it got a score of 5 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its decrease in the overall protein stability as compared to other models.This protein was excluded due to its low QA score as compared to other models. C-beta deviations = 1 , clash score = 45.96 , Molprobity = 2.29 , Ramachandran favored = 98 , Ramachandran outliers = 0.67 , Q mean 4 = -0.54 , Q mean 6 = -0.94.
Figure 1.: Predicted 3D Structure of COH2_GSGS_TD28rev
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