Part:BBa_K4140016
Cas12g
Part Description
RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.
Usage
Cas12g is a RNA-guided protein and differs from other Cas12 proteins by targeting a single strand RNA substrates Making it a potent platform for post transcription modification so we use to control PAH and beta-galactosidase expression as it cleaves the mRNA of PAH and beta-galactosidase at specific site without the need to recognize the PAM Sequence distinguishing it from other Cas proteins preventing the translation PAH and beta-galactosidase just in case of over expression of them or high level of tyrosine and absence of L7Ae as shown in figure 1.
References
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1308
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729
Illegal NgoMIV site found at 1230 - 1000COMPATIBLE WITH RFC[1000]
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