Plasmid

Part:BBa_K4149086

Designed by: Yusang Zhou   Group: iGEM22_Jiangnan-China   (2022-09-30)
Revision as of 12:27, 7 October 2022 by Jet (Talk | contribs)


The plasmid was modified by integrating the cyclopropane fatty acid (cfa )gene with pRSFDuet1 as a v

pRSFDuet1 is an E. coli protein dual expression vector, and we used this as a vector without T7 promoter in order to serve as a control group.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1492
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1492
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1492
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1492
    Illegal NgoMIV site found at 1915
  • 1000
    COMPATIBLE WITH RFC[1000]


0513.png

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Parameters
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