Coding
Part:BBa_K4121019:Design
Designed by: Ziyu Li Group: iGEM22_NEFU_China (2022-10-05)
The HMG-CoA reductase gene HMG1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1311
Illegal PstI site found at 5 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 5
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 685
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1311
Illegal PstI site found at 5 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1311
Illegal PstI site found at 5
Illegal NgoMIV site found at 1456 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.
Source
National Center for Biotechnology Information (NCBI)