Composite

Part:BBa_K4385020

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-06)
Revision as of 09:41, 7 October 2022 by Minan (Talk | contribs)


Zinc-inducible surface display

This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 616
    Illegal NheI site found at 1506
    Illegal NheI site found at 1529
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 616
    Illegal BglII site found at 324
    Illegal BglII site found at 1641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
    Illegal AgeI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None